A team led by researchers from Washington State University used gallic acid (an antioxidant found in gallnut, green tea, and other plants) and applied a stretching mechanism to human chondrocytes taken from arthritic knees. Simulate the stretching that occurs while walking. This combination not only reduces the inflammation markers of arthritis in the cells, but also increases the production of the desired protein normally found in healthy cartilage.
Although still in the early stages, research results indicate that a new procedure can be developed to process cartilage cells extracted from patients to grow cells or tissues for re-implantation.
We found that joint stretching is like an exercise on the cell itself, combined with gallic acid reduces inflammation markers, which means that we can reverse osteoarthritis. It’s basically like good exercise and a good diet on a micro level. ”
For the study, published in Experimental Cell Research, researchers collected osteoarthritis cartilage cells from donated knees taken during joint replacement surgery at Pullman Regional Hospital. They cultivated cells in the laboratory and first tested six antioxidant “nutritions” or nutritional products, including vitamin C, vitamin E, and curcumin. Antioxidants can neutralize free radicals, which are unstable atoms caused by oxidative stress, which can damage cells and tissues.
Laboratory tests show that gallic acid is the most effective antioxidant for neutralizing free radicals in osteoarthritis cartilage cells. Then, the researchers used a cell stretcher developed by Curi Bio Inc. to apply gallic acid and increase the stretch. They set the stretch to 5%, a level that matches the stretch of the knee when a human walks.
This combination reduces inflammation markers called matrix metalloproteinases. It increases the deposition of collagen and glycosaminoglycans, these compounds make connective tissues with integrity, tensile strength and the ability to resist the compressive forces of body weight on joints. Stretching and gallic acid also increased the expression of two other cartilage-specific proteins.
Osteoarthritis is the most common musculoskeletal disease in the world. It destroys the cartilage in the joints, causes pain and restricts movement. There is currently no complete cure. Treatments range from prescribing painkillers to surgery to replace joints with synthetic drugs, but even the surgery does not allow the patient to return to full range of exercise.
Another procedure is called autologous chondrocyte implantation or ACI, which involves removing cartilage cells from the joint, growing them to a large number, and then re-implanting them. The researchers pointed out that at present, the cells are not processed before re-implantation, and lack of treatment can cause the cells to grow weaker fibrocartilage. They may also be affected by osteoarthritis, and these procedures will not restore full function to the joints. This study shows a potential way to develop a similar procedure by first processing chondrocytes and at the same time growing them into tissues that can be re-implanted.
“We are advancing the technology of manufacturing regenerated cartilage in the laboratory. These cartilage may be implanted in cartilage lesions, thereby reducing the number of joint replacements,” said Bernard W. Wojland, a professor in the School of Chemical Engineering and Bioengineering. Said Bernard Van Wie. Principal investigator and corresponding author. “We want to develop a natural cartilage that works normally from the beginning, rather than replacing joints.”
The study adds to the evidence that eating antioxidant-rich foods and exercise may be beneficial, although researchers warn that gallic acid should not be considered a panacea, and any course of action should only be consulted with the person’s doctor.
“This provides some evidence that good diet and exercise are indeed effective,” Abusharkh said. “Even for people with mild osteoarthritis, exercise is really good. Our cartilage tissue is very bad when lying down or sitting all day; we have to do some activities.”
This research was partly funded by the National Science Foundation and the National Health Protein Biotechnology Training Program Institute.
Abu Shak, Ha and others. (2021) Combine stretching and gallic acid to reduce inflammation index and promote the production of extracellular matrix in osteoarthritis human articular cartilage cells. Experimental cell research. doi.org/10.1016/j.yexcr.2021.112841.
Tags: antioxidant, arthritis, bioengineering, biotechnology, cartilage, cells, collagen, consultation, curcumin, diet, doctor, exercise, free radicals, green tea, hospital, inflammation, laboratory, micro, musculoskeletal, bone Arthritis, oxidative stress, pain, pH, protein, research, stress, surgery, tea, vitamin C, vitamin E, walking
In this interview, we talked to Professor Scott H. Faro about his latest research on COVID-19 and its complications in the central nervous system.
Since the beginning of the COVID-19 pandemic, variants have begun to appear. We discussed with Jacob Heggestad about his rapid test that can detect these variants.
In this interview, we chatted with mental health consultant Nella Ciciulla about her daily roles and responsibilities and her career highlights.
News-Medical.Net provides this medical information service in accordance with these terms and conditions. Please note that the medical information on this website is intended to support rather than replace the relationship between the patient and the doctor/doctor and the medical advice they may provide.
Post time: Dec-22-2021
